The EpiIntestinal tissue model (SMI-100) offers numerous advantages versus currently available preclinical testing models such as Caco-2. 基于人体细胞的3D模型包含肠细胞, paneth cell, M cells, 丛细胞与肠干细胞分化成高度分化, polarized epithelium. The EpiIntestinalFT tissue (SMI-100-FT) includes an underlying lamina propria containing normal human intestinal fibroblasts and is useful to study inflammation, wound repair, 先天免疫反应, and fibrosis. Both tissues are cultured at the air-liquid interface (ALI) to allow physiological (luminal) exposure conditions. EpiIntestinal recapitulates many aspects of normal intestinal function including barrier, metabolism, 炎症和毒性反应, 类似于人体的肠道组织.
Important features of EpiIntestinal include the presence of functional tight junctions and brush borders at the apical tissue surface. In vivo, brush borders are in direct contact with luminal contents and include at least 22 metabolic enzymes and 19 drug transporters. EpiIntestinal tight junctions and brush borders closely mimic human structure and physiology.
The EpiIntestinal 3D human tissue model is routinely utilized for screening pharmaceuticals for a range of endpoints including GI toxicity and inflammation, drug metabolism, drug permeation, fibrosis, 以及肠道感染. Straightforward protocols and the evaluation of early molecular and cellular endpoints often allow scientists to acquire data in days, not weeks or months.
Use EpiIntestinal for a highly predictive assay to evaluate GI toxicity of pharmaceuticals.
Utilize EpiIntestinal’s highly controlled in vitro culture conditions to study intestinal inflammation, wound repair, 并评估治疗效果.
Drug Delivery & Metabolism
Utilize EpiIntestinal for highly predictive and human-relevant drug delivery. 评估原料药的生物利用度(内流)和外排运输, drug metabolism, 以及药物-药物相互作用. 上皮肠可与其他生理系统(如.g. liver cells) in microfluidic systems to examine the effect of drug metabolites on GI toxicity or to predict drug permeation/absorption via the “luminal” side of the tissue.
Use EpiIntestinalFT to better understand mechanisms of wound healing in the gut and to evaluate therapeutic effects.
应用EpiIntestinal研究胃肠道感染, host-pathogen交互, 先天免疫反应.
Browse our reference library to learn more about how researchers have used our EpiIntestinal tissue in these areas of study.
Kit: SMI-100和SMI-100- ft EpiIntestinal试剂盒由24个个体组织组成. SMI-196 or SMI-196-FT EpiIntestinal kits consist of 96 tissues in a high throughput screening plate. Each kit contains tissues, culture medium to support experiments for 24 hours, and plasticware. 请联系bbin体育官方网站以获取特定的套件内容.
- SMI-100 and SMI-100-FT: Individual tissues cultured in cell culture inserts.尺寸:9毫米组织直径(表面积= 0.6 cm2 )，底层微孔膜孔径= 0.4 μm
- SMI-196 and SMI-196-FT: Tissues grown in 96-well high throughput screening plate. Dimensions: 0.19厘米组织直径(表面积= 0.11 cm2). 底层微孔膜孔径=0.4 μm
Lot numbers: 每周生产的纸巾批次都有一个特定的批号. A letter of the alphabet is appended to the end of the lot number to differentiate between individual sets of 24 tissues within a given production lot of tissues. 一个生产批次内的所有组织试剂盒在细胞方面是相同的, medium, handling, culture conditions, etc.
Shipment day: Every Monday
Delivery: 周二上午通过联邦快递优先服务(美国). 美国以外:周二至周三，视地点而定.
Shelf life: Including time in transit, tissues may be stored at 4°C for up to 2 days prior to use. However, the best reproducibility will be obtained if tissues are used consistently on the same day, e.g. Tuesday afternoon or following overnight storage at 4°C (Wednesday morning).
实验时长: Tissue cultures can be continued for ONE (1) MONTH or more with good retention of normal morphology. 纸巾必须每隔一天喂5次.0毫升维护培养基(SMI-100-MM). Cell culture inserts are placed atop washers (EPI-WSHR) or culture stands (MEL-STND) in 6-well plates to allow use of 5.0 ml.
Genetic make-up: Single donor
Derived from: Healthy adult donor
Screened for: HIV，乙型肝炎，丙型肝炎，支原体
Base medium: Dulbecco 's Modified Eagle 's Medium (DMEM)
Antibiotics: Gentamicin 5 µg/ml
Anti-fungal agent: Amphotericin B 0.25 µg/ml
pH Indicator: Phenol red
Other additives: Proprietary
Alternatives: Phenol red-free, antibiotic-free, anti-fungal-free medium and tissues are available. 代理商至少在发货前3天被移除.
Visual inspection: 所有组织都经过目视检查，如果发现物理缺陷, 纸巾被拒绝装运
Sterility: 在整个生产过程中使用的所有介质都要进行无菌检查. Maintenance medium is incubated with and without antibiotics for 1 week and checked for sterility. The agarose gel from the 24-well plate used for shipping is also incubated for 1 week and checked for any sign of contamination
病原体筛查: All cells are screened and are negative for HIV, hepatitis B and hepatitis C using PCR. However, no known test method can offer complete assurance that the cells are pathogen free. Thus, these products and all human derived products should be handled at BSL-2 levels (biosafety level 2) or higher as recommended in the CDC-NIH manual, 微生物和生物医学实验室的生物安全,” 1998. For further assistance, please contact your site Safety Officer or bbin体育官方网站 technical service
不合格批件通知: 如果纸巾批次没有通过质量控制或无菌检测, the customer will be notified and the tissues will be replaced without charge. 因为我们的QC和无菌检测是在出货后完成的, 在一般情况下，我们会尽快发出通知, ET-50故障将在周四下午5点前通知.m.; sterility failures will be notified within 8 days of shipment)
To ensure that the most appropriate products and accessories for your application are included with your initial order, bbin体育官方网站 strongly recommends a complimentary consultation with one of our highly trained Technical Specialists. 在美国，请联系Alex Armento订购EpiIntestinal产品. 在欧盟，请联系Dr. Jan Markus.
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